Overview
(organisation, research areas, research objectives, etc.)
Institutional reform, research potential European integration Significant results
Research laboratories and teams
BRIEF HISTORY
1895
1926
1948
1970
1981
2005
2013
IZ – Research Institute for Animal Husbandry (First in Europe)
INZ – National Institute for Animal Science
ICZ – Research Institute for Animal Science
ICNA – Research Institute for Animal Nutrition
IBNA – Research Institute for Animal Biology & Nutrition
INCDBNA –National Research-De- velopment Institute for Animal Biology and Nutrition, under AAFS coordination
better adaptability / higher standards
than all other institutes in animal science
- good premises for core funding
INCBNA – National Research-
Development Institute for Animal Biology and Nutrition, under MNE coordination
developing IBNA brand
- 5 -
IBNA ORGANISATIONAL CHART
ADMINISTRATION COUNCIL BOARD OF DIRECTORS
SCIENTIFIC COUNCIL
DIRECTOR OF SCIENCE RESEARCH
GENERAL MANAGER QUALITY MANAGEMENT COMPARTMENT
LABORATORY OF ANIMAL BIOLOGY
LABORATORY OF CHEMISTRY AND NUTRITION PHYSIOLOGY
LABORATORY OF ANIMAL NUTRITION AND
BIOTECHNOLOGIES
TECHNICAL DIRECTOR DEVELOPMENT
PILOT STATION FOR PROTEIN- VITAMIN-MINERAL CONCEN- TRATES AND COMPOUND FEEDS
MANUFACTURE
EXPERIMENTAL CROP AND LIVESTOCK FARM
TECHNICAL ASSISTANCE DEPARTMENT LABORATORY FOR QUALITY INSPECTION
ECONOMIC DEVELOPMENT DIRECTOR
ADMINISTRATION
BUDGET AND FINANCIAL ACCOUNTING DEPARTMENT
MARKETING COMMERCIAL, TRANSPORT AND EXTENSION
DEPARTMENT
ORGANIZATION, HUMAN RESOURCES AND WAGES
DEPARTMENT
ADMINISTRATION, P. R., LEGAL, AUDIT DEPARTMENTS DOCUMENTATION, EXTENSION
LABORATORY OF ANIMAL BIOLOGY
RESEARCH TEAMS
Feed safety and immuno-nutrition
6 scientists Coordinator Ionelia ȚĂRANU
Animal Genetic Resources Management
6 scientists Coordinator Horia GROSU
LABORATORY OF CHEMISTRY AND NUTRITION PHYSIOLOGY
LABORATORY
OF ANIMAL NUTRITION AND BIOTECHNOLOGIES
Animal Nutrition and Biotechnologies
6 scientists Coordinator Mihaela HĂBEANU Physiology of ruminant
animals nutrition 6 scientists Coordinator Cătălin DRAGOMIR
Physiology of monogastric animals nutrition
6 scientists Coordinator Rodica Diana CRISTE
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RESEARCH AREAS – KEYWORDS
1. FEED SAFETY AND IMMUNO-NUTRITION
# Feed safety (mycotoxins)
# Intestinal barrier (intestine) # New feed sources / immune response
# Phyto-additives / immunity
# Nutrient bioavailability
# Quality of feed & animal products
# Animal nutrition &
the environment
# Functional foods
# Efficiency of rumen metabolism
# Feeding values (tables, prediction)
# Subacute rumen acidosis
# Rumen manipulation
# Feed sources / feeding efficiency
# Animal welfare / environment
# Nutrition & organic production systems
# Feed additives / ensiling with lactic acid bacteria
# Breeding programs
# Biometry & genetic potential for reproduction
# Vulnerable & endangered populations
# Genetic markers for selection
2. ANIMAL GENETIC RESOURCES MANAGE- MENT
3. PHYSIOLOGY OF MONOGASTRIC NUTRITION
4. PHYSIOLOGY OF RUMINANT NUTRITION
5. ANIMAL NUTRITION AND BIOTECHNOLOGIES
SOME FIGURES
155 employees: 48 in research, 75 in development, 32 in administration. Of these 31 are researchers - 21 PhD in sciences + 7 doctoral students
- 3 senior researcher I, 9 senior researcher II, 8 senior researcher III, 7 researchers, 4 research assistants
- Average age: 43
- >40% = studies abroad (PhD, post-doc)
Balance, stability, outstanding potential for 2014-2020
>21 billion RON turnover
(highest among AAFS units – 2011, 2012, 2013, 2014)
Intense activity to capitalise on the research outputs - Dozens of commercial contracts each year
- Professional relations with dozens SMEs each year - Professional relations with almost all associations from animal production and related sectors
30-40 research projects running each year (of which 5-10 international) => Dozens of results each year – applied (towards farmers, processors, etc.)
- fundamental (ISI papers)
=> Interaction with dozens of animal production companies + related sectors => High visibility + European relevance (SE Europe, ERA)
Many activities of technological transfer each year, in different ways (classical, modern), financed from own resources.
2020: clear strategy to enhance the role of supplier of scientific knowledge at the national and international levels
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STRENGTHS
Timely accomplishment of the institutional reform aiming to increase the role of the institute within the animal science sector and to increase its integration within the Europe- an Research Area (organisation, research directions, project running, technological transfer efficiency, etc.)
High international visibility – by the large number of international projects in which it participated (such as FP6/506144,
FP6/043077, FP6/506087, FP7/207043, FP7/266061, FP7/266367); relevance of the international collaborations/ in workgroups, commissions, etc./- flow of scientific publications and communications
Capacity to approach both fundamental research (relevant for the international scientific environment) and applied research, whose
outcomes can be rapidly transferred in the agricultural production (during the progress of the research projects)
High proportion (over 50%) of researchers with significant specialisations abroad (PhD, post-doc), who developed relations with the host institutions and who perform at international standards
High competitional capacity/
adaptability/ speed of reaction
Broad network of collaborations with the potential beneficiaries of the research results (farmers, professional associations, authorities, etc.), which ensures the applied relevance of the research directions and the high impact of the research outputs.
Position within animal science branch of the Academy of Agricultural and Forestry Sciences (A.A.F.S.)
- > 60% of the projects = in IBNA
- > 60% of the results (~ 80% of the ISI articles) = in IBNA - Almost all international projects = in IBNA
- Most of the international activity = in IBNA
Position within A.A.F.S.
- Highest financial turnover in 2010 and 2011
- Most intense international activity (projects, articles, events, trainings, etc.)
Position within the Romanian agricultural research - The only national institute in animal science sector - One of the highest loads of projects / researcher - The highest degree of integration within the E.R.A.
- The highest number of international projects and of researchers involved in international collaborations
Vision & reform &management strategies
OUTCOME OF THE EVOLUTION FROM 2003 TO 2014
TURNOVER AND THE RESEARCH BUDGET
Important: the institute never had debts to the state budget or to the local budgets.
102 128
157 165 165 182
204 220 206
236
0 50 100 150 200 250
2005 2006 2007 2008 2009 2010 2011 2012 2013 2014
millions lei
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STRATEGIC OBJECTIVES 2003-2014
STRATEGIC OBJECTIVES 2014-2020
TWO MAIN DIRECTIONS
FINALIZING THE INSTITUTIONAL REFORM, MAKIS PROJECT
(financed by MADR in collaboration with the World Bank)
INTENSE RESEARCH ACTIVITY, WITH TWO FOCUSES:
- personnel - infrastructure
- evaluation & monitoring - research priorities
- European priorities E.R.A.
- supporting the Romanian animal science sector
1. Institutional development so that IBNA turns into a relevant European actor 2. Increasing IBNA impact on the Romanian animal science/ animal production sector (research directions, technological transfer)
3. Technical and scientific support for the authorities and for the private sector to solve the challenges of the 21st century in the field of animal production
- Identification of new resources within the context of limited conventional resources
- Necessity of maintaining food security and safety - Climate changes
- Counteract the competition of the emerging economies (China, Brasil, Argentina)
- Decrease the dependence on imported protein feeds
- Adaptation to the evolution of the industries which use forages as raw materials (biofuels)
- Alleviating the environmental impact of animal production - Ensuring animal health and welfare
- Adaptation to consumer requirements (quality and safety), including target groups, functional foods
OUTCOMES OF THE REFORM RESEARCH POTENTIAL
Human resources reform
- Optimal average age and structure of the staff (stability, potential, promising careers)
- Ability to train the newcomers (in the institute / in the country / abroad) - IBNA = attractive for early career researchers (research methods unique in Romania, international opportunities) - Mechanisms for the development of scientific careers
- Good potential for 2014 – 2020
Scientific reform
- 3 PhD degrees obtained in France - 5 post-doc trainings in France (8 years in all), Belgium, USA, etc.
Trained in IBNA and abroad for short periods (1-3 months in Netherlands, Belgium, France, Germany etc.)
Licenced technicians and laboratory workers
- Post-doc training in Japan, France, Luxemburg
- PhD obtained in France - 1-3 months of training in
Switzerland, France, Belgium, Germany etc.
AUXILIARY STAFF ATTESTED RESEARCHERS SENIOR
RESEARCHERS
NEWCOMERS
Over 40% of the special training periods
were on medium &
long periods Almost permanently researchers are abroad
for training
Research priorities adapted to the national and international requirements
1 2 3 4 5 6 7
Project-structured activity (competitiveness, efficiency, monitoring)
IBNA = attractive for the potential collaborators (local/foreign); for instance feeding trials, trials at rumen level, immune-feeding, nutrient bioavailability (for various category of farm animals) – many offers for project proposals resulted
Two-fold higher indicators showing the results of the research activity (ISI articles, patents, etc.)
High capacity to submit project proposals
Modernization and enlargement of the projects portfolio Good participation in EU (FP, etc.) and national programs
OUTCOMES OF THE REFORM RESEARCH POTENTIAL
Modernization of the infrastructure
- Laboratory equipment purchased through MAKIS and through own projects
- Research equipment worth more than 1 million Euros purchased during the recent years
- Research equipment single in Romania / rarely met in SE Europe (digestibility cages, in vitro digestibility, etc.)
- Complete infrastructure for research into the biology and nutrition of farm animals (arable land + specific equipment / compound feeds mill, experimental crop and livestock farm – ensuring the autonomy of the specific researches) - Good coverage of the infrastructure with staff, research methodology, funds for research, etc.
- Very high level of using the infrastructure (large number of projects, research outcomes, etc.)
SCIENTIFIC RESULTS – OPPORTUNITY
PRODUCTS AND SERVICES
- High flow of the research outcomes from research projects
- Complex approach – results adapted to the different types of beneficiaries - wide range of TT means
I.B.N.A.
Active TT
- Farm demonstra- tions
- Training - Seminars - Exhibitions, fairs
Passive TT - Publications - Communications - Reports
- Patents - Brochures, guidebooks Technological
transfer (TT)
feedback
Dozens of products, outcomes of the R&D projects:
- CPVM-BNA PCD 35;
- NC AGN-3 “Eco”
- Proteostim - CPVM 2S pig - NC AGPN
- EurOU / NC EurOU - ...
Dozens of services resulting from R&D projects:
- Fine chemical composition - Feeding value
- Rumen degradability - Digestibility
- Diet optimization - Feed trials - ...
Targets of the technological transfer
- Farmers - Processors
- Feed manufacturers - Associations
- Feed additives companies - Through territorial G.O.
- ...
TT contracts
Market launch
Public information
Project partners
Forms of technological transfer:
- Brochures, fliers, guidebooks - Demonstration activities - Fairs / exhibitions
- Website posts / newsletters - Mass-media
- ...
- 15 -
TRANSFER ACTIVITIES MATERIALISED IN:
JUST TWO EXAMPLES
- Very efficient / rapid way of transfer of scientific results - High impact within the private sector / national visibility
Transfer activities materialised in:
- Good national impact of the research outcomes
- Capacity to draw increasingly higher funds from the private sector (such as special analyses: amino acids, van Soest, degradability, feeding value, immune status, myco- toxins, microbiology, etc.)
- Policy of maintaining a high impact on the private sector
IBNA project Private partner
EUREKA Emona Nutrition R&D Department, Slovenia SUPLIBOR SC Natural Research Craiova
FITOMIN SC Hofigal SA
EUREKA SC Avicola Bucuresti SA OMEGANUTRIOSAN SC Avicola Bucuresti SA
FITOMIN SC Transapicola SA
METAVIAC S.C. SIAT S.A.
FP7 SOLID (Task 3.1) Agro Solomonescu SRL PS 7.1.3. Societatea Ecologica Aquaterra
PS 7.1.3. SC Kaviar House Bucuresti - Filiala Tulcea SRL
AVIECO SC AugerPetrus SRL
FP7 WOOLVATION Centiv GmbH, Germania (proposal) BYPROD_RUM Ilya Alexandria (proposal)
R&D contract Private financer Contr. 908/13.02.2007 SC AGRALIM SA
Contr. 1166/29.02.2008 SC Mibos Project SRL Campulung, Arges Contr. 5740/30.10.2009 SC TOP NUTRITION
Contr. 1598/26.03.2010 SC CAN 2000 TRADING SRL Contr. 3373/30.06.2010 SC Exploatarea Miniera Harghita Contr. 4358/20.08.2010 SC Exploatarea miniera Harghita Contr LNA/01.01.2010 Semar Trading SRL
Contr. 2125/4.05.2011 SC ANGST RO-SA Contr. 2154/05.05.2011 Banvit Food SRL
Contr. 2245/10.05.2011 SC Exploatarea Miniera Harghita Contr. 2831/8.06.2011 Heidi Chocolat SA
Contr. 3422/5.07.2011 SC AGROMAR SRL Contr. 3715/18.07.2011 AGCTR
Contr. 3373/01.01.2012 AGCTR
Contr. 5076/21.09.2011 SC Exploatarea Miniera Harghita
EXAMPLE OF PARTNERSHIP WITH THE PRIVATE SECTOR
Development of projects on the topic of eggs as functional food
CEEX Program OMEGANUTRIOSAN
project
(Omega 3 eggs on the Romanian market)
1 EUREKA project making use of eggshells 1 INNOVATION project
low-cholesterol eggs
1 EUREKA project natural
antioxidants ω 3 eggs
1 INNOVATION project
egg with xantofilic pigments
Nucleus Program Project PN 09-38 04.01
Using the camelina meal and grape mark rich in antioxidants to improve pig
meat. Development of projects on subject of using
industrial by-products and wastes to feed farm animals
Development of projects on the topic of meat as functional food SC VINARIJA
SIJACKI, SERBIA Avicola Lumina S.A.
Slovenia
Project MADR/59/7.2.1 –
Partnership Sectoral Program
ADER 2020 SC. SUINPANAGRO -
SRL Dambovita
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SIGNIFICANT RESULTS
Updating the farm animal feeding system: feeding norms, feeding values tables, modelling the metabolic
processes (1995-2000)
Updated feeding system
- Feeding norms, feeding value tables, diet optimization, etc.
- It integrates 10 years of scientific accumulations
- Widely disseminated (guidebooks, technical books, articles, tables, communications);
taught to the students learning animal husbandry
- This feeding system is used to develop and update permanently over 80 formulations of standard feeding products of the institute (premixes, protein-vitamin-mineral concentrates, compound feeds)
Technologies/formulations/products to replace animal feeds
- IBNA has 10-15 products containing replacers of the animal feeds, which allowed to maintain the economic efficiency
- IBNA was the main source of technical solutions during that period Examples: feeds based on rapeseed meal, full-fat soya, etc.
Feedstuffs used to enhance the quality of animal products and farm competitive- ness, within the context of integration on the European market (2005-2010)
Modernising the genetic breeding programs for farm animals: use of genetic markers, of new mathematical models, etc. (2005-…)
Set of technologies/formula-
tions/products to solve the efficiency problems caused by the ban on animal protein feeds (2000-2005)
Feeding strategies for alternative production systems (ecologic,
traditional), niche products, alleviate the environmental impact, etc. (2000-…)
Solutions to adapt animal production to the challenges of the 21st century>
fewer resources, harsher competition, climate changes, globalization (2010-…)
SIGNIFICANT RESULTS
Technologies/feed formulation/feed products/norms to support the farmers within the context of European integration (= food quality and safety problems, competitiveness, environmental issues, etc.)
- Integration opened the way for direct competition between the Romanian and European animal producers
- The institute researched, developed and disseminated over 100 products, techniques, feeding strategies and norms:
Example: products based on camelina meal; safflower meal; corn distiller grains; grape marc, products rich in antioxidants, omega 3 and omega 6 polyunsaturated fatty acids.
The feed products are patented or patent applications have been submitted, ex.:
A/00065/2004; A/00822/2006; A/00550/2011; A/00207/2012; A/00231/2012) - Low-cholesterol eggs
- Eggs, pig meat and cow milk enriched in omega 3 and omega 6 polyunsaturated fatty acids
- Techniques to alleviate acidosis in cows - Norms for mycotoxins
The systematic popularization of these results allowed their large scale generalization – MANY OF THEM ARE NOW INTEGRATED IN PRACTICAL ANIMAL PRODUCTION Genetically and economically optimized breeding programs for farm animals
- The implementation of these results may ensure an average 1-2% annual increase of the genetic potential, provided the other factors (feeding, microclimate, etc.) are supplied at a proper level
- Within the current context (unsatisfactory state of the genetic resources) this increase of the genetic potential can be even higher, which means additional economic advantages.
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PUBLICATIONS
(2012 AT THE MOMENT OF ACCREDITATION)ISI journals:
Brith J. Nutr.
Anim Sci J.
Animal
Toxicol in vitro J. Agric.
Food Chem.
Mol Nutr Food Res.
Meat Sci.
Toxicon
J Trace Elem Med Bio.
ISI articles / of which non zero influence score
IDB indexed articles International communications
Indexing in databases:
CABI – full text products PROQUEST
EBSCO IVIS ULRICH
SPRINGERLINK + on the way ...
Conferences/
symposia organized by IUPAC,
Nutrition Society, WPSA,
EAAP
average
2002-2006 2007 2008 2009 2010 2011 average 2007-2011 2.5 / 1.2 2 / 2 4 / 1 4 / 4 7 / 7 8 / 5 5 / 3.8
9.5 7 23 5 15 10 12
14.0 11 22 16 23 26 19.6
GOOD POTENTIAL
2012-2020 FOR
The analysis doesn’t include the scientific events organised in Romania (even if they were international)
Growing trend for all indicators
Best performance in agricultural research (productivity/researcher)
INTERNATIONAL PROJECTS
(ACREDITATION 2012)2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50 52 54 56 58 60 62 64 ...
FP6 Feed for Pig Health FP6 FeedSEG
FP7 Feed to Food ----> 2012
FP7 SOLID ----> 2016
FP7 FoodSEG ----> 2014
bliateral Ro-Slovenia ECO-NET
bilateral Ro-France (bis) RFR (bis)
COST FA0802 ----> 2012
Eureka ----> 2013
bilateral Ro-Greece ----> 2014
2011 2012 and on
2007 2008 2009 2010
- Proven potential for participation in FP projects - Experience (both FP logistics / scientific)
- Good image / visibility (warranties from former coordinators, repeated invita- tions to join consortia, etc.)
- IBNA = very open for ERA / swiftness / seriousness
- IBNA = manages to draw each year research funds from European research programs
EUROPEAN INTEGRATION
Developed European projects, examples:
FP7 “Solid”
FP7 "FOODSEG"/2011-2013
IBNA-MAICH bilateral Grecia MICOEX/2011-2013 COST-FA 0802/ 2008-2013
FP7 "FEED-TO-FOOD" /2008-2011
IBNA-INRA bilateral Franta "Reseau Formation researche"/
2006-2009
FP6 "Food for pig health"/2004-2008
Developed European projects, examples:
IBNA – France bilateral H2020 TEAMING – “BNA-CE”
H2020 – GeNuM BBI “RO – by-products”
- Special focus on FP6 - FP7 (... FP8, H2020); Other kinds of projects
= depending on the availability /efficiency /particular interest
FP7 SOLID 2011-2016 www.solidairy.eu
FP7 Feed to Food =>
FOODCLUSTER 2008-2012
FP6 Feed for Pig Health 2004-2007 (The participation in
FeedSEG allowed IBNA to be invited in FoodSEG
consortium) FP7 FoodSEG
2011-2014
- 21 -
LABORATORY OF ANIMAL BIOLOGY (LAB)
Team 1: Food safety and immunonutrition Team coordinator: Dr. Biol. Ionelia Taranu
Team 2: Genetic resources management Team coordinator: Prof. Dr. Ing. Horia GROSU
LAB INFRASTRUCTURE
COMPARTMENT FOR IN VITRO CELL CULTURE:
- Primary cells (lymphocytes, monocytes, neutrophils) from blood and immune organs (spleen, lymph nodes) - Cell lines (intestinal epithelial) – pigs and humans (IPEC-1, Caco2, LLCPK, etc.)
COMPARTMENT OF MOLECULAR BIOLOGY:
which analyses the gene expression of different
biomarkers playing an important role in the metabolic and physiologic processes from cells and tissues (blood, organs, muscle, milk);
genes for:
- Cytokines - Enzymes
- Signalling molecules - Proteins
Developed European projects, examples:
IBNA – France bilateral H2020 TEAMING – “BNA-CE”
H2020 – GeNuM BBI “RO – by-products”
Team 1 2-SR: I 2-SR: II 2-SR: III 1-ACS
1-Technician Ionelia
ȚĂRANU SR I
Daniela MARIN SR I
Mihai PALADE SR GinaPISTOL
SR II
Veronica CHEDEA SR II
Monica MOȚIU ACS
Mariana STANCU tehnician
Horia GROSU Profesor SRI
Elena GHIȚĂ SR II
Rodica PELMUȘ SR III
Cristina LAZĂR SR III
Mihai GRASSR III
Loredana CĂLIN tehnician
Cătălin ROTAR ACS Team 2
1-profesor-SRI 1-SR: II 3-SR: III 3-SR1-ACS 1-Technician
SR=scientist researcher
LBA INFRASTRUCTURE
LAB – ANALYSES FOR THIRD PARTIES
COMPARTMENT OF BIOCHEMISTRY (PROTEOMIC)
which investigates (using biochemical
techniques, electrophoresis, western blot and ELISA) the profile of blood, cell and tissues proteins:
- Immunoglobulins - Cytokines
- Signalling molecules - Other proteins
Micotoxicology:
-ELISA -TLC
Testing products with biological potential:
- in vitro on pig and human cells - in vivo on pigs, poultry, dairy cows Performance and immune response (antibodies, cytotoxicity, cell proliferation, immunoglobulins, cytokines, gut barrier integrity, etc.)
Blood metabolic profil COMPARTMENT OF
PHYTOCHEMISTRY
which investigates different bioactive com- pounds (such as polyphenols) from natural extracts and from forages determining their antioxidant activity, the UV-Vis spectral print and the inhibition of pro-oxidizing enzymes (such as lipoxygenase, etc.).
Analisys Cost (lei)
Total aflatoxin 65.00
Aflatoxin B1 65.00
Ochratoxin A 65.00
Zearalenon 65.00
Fumonisins 65.00
Deoxynivalenol 65.00
Analisys Cost (lei)
Albumin 8.00
Total bilirubin 7.00
Phosphorus 14.00
Calcium 7.00
Iron 9.00
Sodium 9.00
Magnezium 7.00
Potassiu 10.00
Glucose 7.00
Total cholesterol 7.00 Triglycerides 9.00
IgG 18.00
IgM 18.00
IgA 18.00
Creatinine 7.00
Urea 7.00
Total Protein 8.00
Gama GT 10.00
TGO 7.00
TGP 7.00
LF INFRASTRUCTURE EXPERIMENTAL HOUSES FOR POULTRY/PIGS
- 23 -
LABORATORY OF CHEMISTRY AND NUTRITION PHYSIOLOGY (LF)
Experimental facilities for:
- digestibility
- mineral metabolism
- quality of animal products (meat, eggs)
- environmental effect of animal production
- effect of various factors (microcli- mate included) on animal produc- tion-efficiency of feed utilization - etc.
Team 3: Nutrition physiology of monogastric animals Team coordinator: Dr. Rodica Diana CRISTE
Team 4: Nutrition physiology of ruminant animals Team coordinator: Dr. Cătălin DRAGOMIR
Team 3 2-SR:I 3-SR:II
1-SR Rodica
Diana CRISTE SR I
Margareta OLTEANU SR I
Iulia MAROȘ SR Mariana
ROPOTA SR II
Arabela UNTEA SR II
Tatiana PANAITE SR II
Team 4 2 SR:II 2 SR:III
1 SR1 AC Cătălin
DRAGOMIR SRII
IlieVOICU SRII
Smaranda TOMASRIII
AnaCIȘMILEANU SRIII
Andreea VASILACHI SR
Eugenia MIRCEA AC SR=scientist researcher
LF INFRASTRUCTURE
EXPERIMENTAL HALLS FOR RUMINANT ANIMALS
LABORATORY FOR PHYSICAL-BIOCHEMICAL ANALYSES (RENAR ACCREDITATION, SANITARY-VETERINARY LICENCE)
Used for trials to determine the:
- rumen metabolism (rumen degradability, environmental effect of the different diets/efficiency of nutrient utilization, manipulation of rumen processes), - apparent forage digestibility, - feeding value for ruminants, - quality of animal products
Basic analyses:
- determination of the basic chemical com- position and of the feeding value,
- dynamics of pH and of rumen ammonia, - determination of egg quality
- in vitro digestibility (ruminants) In-depth analyses (chromatography, spectrometry):
- content of vitamins/amino acids/minerals of the forages, animal products, biological tissues and fluids,
- cholesterol concentration of animal prod- ucts,
- metabolites relevant for animal feeding, - quality of fat,
- contaminants
- 25 -
EQUIPMENT AND METHODOLOGY
MONOGASTRIC ANIMALS RUMINANTS
- fistulised animals (cows, sheep) - digestibility stands/pens
- in vitro system (anaerobiosis, thermal stirrers, centrifuge)
- equipment for in sacco incubation - ion-meter (rumen pH, ammonia) - GC – fatty acids and volatile fatty acids - HPLC – amino acids
Dumas system for nitrogen determination Rumen degradability
Rumen pH dynamics
Milk fatty acids profile
Apparent digestibility
Apparent digestibility
Microbial protein synthesis (biomarkers)
Rumen ammonia dynamics profile VFA dynamicsand
- efficient utilization of feed resources (by-products, natural feed additives) - nutrient metabolism (minerals, etc.) - decrease the environmental impact of animal production
- production of functional foods - quality of animal products (eggs, meat)
- contaminants – heavy metals Equipment for egg
quality determination
Experimental halls for poultry/pigs
Digestibility cages fir poultry/pigs
Fatty acids profile
Amino acids profile
Proximal analyses
Mineral profile Contaminants
(Pb, Cd) Fat quality
(acidity, peroxides)
The prices are shown at www.ibna.ro, section of “Products and Services – Physical, chemical and biochemical analyses”
LF – ANALYSES FOR THIRD PARTIES FROM FORAGES, ANIMAL PRODUCTS (MEAT, MILK EGGS), BIOLOGICAL TISSUES AND FLUIDS
Analysis
Sample preparation for analysis SR ISO 6498 – 2001 Determination of moisture content gravimetric method
Determination of nitrogen content mineralization and steam distillation Determination of fat content extraction with organic solvents Determination of crude fibre content method with intermediary filtration Determination of total ash gravimetric method Determination of urease activity volumetric method
Kreiss reaction STAS 12266 – 84
Fat acidity indicator volumetric method
Peroxide indicator volumetric method
Determination of calcium content atomic absorption spectrometry
Determination of Cd and Pb content graphite furnace atomic absorption spectrometry (GF-AAS)
Determination of phosphorus content spectrophotometry Determination of fatty acids content gas chromatography
GC cholesterol gas chromatography
– AOAC 994.10 and ISO 12228 - 1999 Determination of protein, fat and lactose infra red spectrometry –
(MILKO SCAN 605) AOAC 972.16; International
IDF Standard 141C - 2000
- 27 -
LF – ANALYSES FOR RESEARCH CONTRACTS
Fat quality
Contaminants
Egg quality
Nutrition physiology
Analysis
Free acidity Kreiss reaction Fat acidity indicator Peroxide indicator
Volumetric method
Lead (Pb), Cb (cadmium) -
graphite furnace atomic absorption spectrometry (GF-AAS)
Weight of egg components (egg white/yolk/shell) Egg shell thickness
Egg shell breaking strength Egg freshness
Egg yolk colour intensity Haugh unit
gravimetric method physical measurement electrometric method physical measurement
Van Soest method Van Soest method
High Performance Liquid Chromatography (HPLC) 1. Preparation of methyl esters; 2. Gas chromatography Gas chromatography
Atomic absorption spectrometry Photometry
Flame emission spectrometry Atomic absorption spectrometry Volumetric method
Electrometric method Polarimetric method Volumetric method Gravimetric method
Analysis
NDF
ADF
Amino acids profile (15 amino acids) Fatty acids profile (21 fatty acids)
Cholesterol
Calcium
Phosphorus Minerals (sodium, potassium, magnesium) Trace minerals (iron, copper, manganese, zinc) Urease activity
pH of watery solutions
Starch
Total chlorides HCl insoluble
}
EXPERIMENTAL ANIMAL FARM - arable land for experimental crops
- pilot station for experimental compound feeds - housing facilities for cattle / sheep / pigs / poultry adapted for feeding trials
- forage stock (silages, hays)
LAN INFRASTRUCTURE
Laboratory of animal nutrition and biotechnologies (LAN) Head of laboratory: Dr. Eng. Mihaela Habeanu
Team 5 3 SRII 2 SRII
1 SR Mihaela
HĂBEANU SR II
Dorica VOICU SR II
Nicoleta LEFTER SR Cristina
TABUC SR II
Georgeta CIURESCU SR III
Anca GHEOR- GHESRIII
gravimetric method physical measurement electrometric method physical measurement
Van Soest method Van Soest method
High Performance Liquid Chromatography (HPLC) 1. Preparation of methyl esters; 2. Gas chromatography Gas chromatography
Atomic absorption spectrometry Photometry
Flame emission spectrometry Atomic absorption spectrometry Volumetric method
Electrometric method Polarimetric method Volumetric method Gravimetric method
Analysis
NDF
ADF
Amino acids profile (15 amino acids) Fatty acids profile (21 fatty acids)
Cholesterol
Calcium
Phosphorus Minerals (sodium, potassium, magnesium) Trace minerals (iron, copper, manganese, zinc) Urease activity
pH of watery solutions
Starch
Total chlorides HCl insoluble
SR=scientist researcher
- probiotics from lactic acid bacteria and yeasts tailored for the main species of farm animals (Avilact, Suinlact, Bovilact, Ovilact)
- ensiling additives from lactic acid bacteria (Biocons)
These products can be fed directly to the animals or for ensiling, thus contributing to the efficiency of feed utilization, to their sanitation, etc.
PILOT STATION FOR BIOTECHNO-
LOGICAL PRODUCTS
Composed of:
Section for the manufacture of vitamin-mineral premixes, vitamin- protein-mineral concentrates, technical assistance
Section for compound feeds manufacture Laboratory for quality check
Functional annexes: storing facilities, fuel tanks, boiler room, mechanic shop, water tank
Technical Director
Pilot station for experimental feeds
TECHNICAL AND DEVELOPMENT DEPARTMENT
PILOT STATION FOR COMPOUND FEDS
Head of QC laboratory
Experimental crop and animal farm
Manufactures the variants of compound feeds used by the research projects run by the research
laboratories
These products are intended for all the species and categories of farm animals
Contributes to the technological transfer by selling a wide range of specific products developed through research on animals
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PILOT STATION FOR COMPOUND FEED MANUFACTURE
EXPERIMENTAL CROP AND ANIMAL FARM
The number and structure of the formulations developed by the institute vary very much, depending on the outcomes of the scientific research, market demands, etc.
However, each year there is a basic number of formulations as follows:
28
standard formulations of vitamin-protein-mineral concentrates (CPVM) (17 for poultry, 6 for pigs and 5 for ruminants)37
standard formulations of compound feeds (23 for poultry, 6 for pigs and 8 for ruminants)20
standard structures of vitamin-mineral premixes (zoofort) (12 for poultry, 3 for pigs and 5 for ruminants)- medicated premixes
- biopreservatives, probiotics (Biocons, Avilact, Bovilact, Suinlact)
The formulations can be tailored according to the requirement of customers (research laboratories, other research institutes, economic enterprises, farmers)
Provides support for the research projects conducted on all species and categories of farm animals
Facilities:
237 ha of irrigable arable land used for experimental crops
Housing facilities for pigs, cattle, sheep and poultry adapted for experimental activities:
- cattle: tie-stall cows, free-stall cows, fattening steers, profilactorium, glass fibre pens for calves, summer paddock
- sheep: pregnant sheep, lactating sheep and lambs, fattening lambs - pigs: maternity hall, growing pigs, pregnancy – boar hall, fattening pigs - poultry (various species): battery or deep litter systems
Platform for forage processing and ensiling
Functional annexes: sanitary filter, headquarters, boiler room, mechanic shop
