COMPARISON OF VOLATILE COMPONENTS OF STACHYS
LAVANDULIFOLIA VAHL OBTAINED BY MWHD AND HD TECHNIQUES
aDepartment of Physics, University of Guilan, Rasht PO Box 41365-1159, Iran
bDepartment of basic engineering, Qom University of technology Qom, Iran ,M.H.MESHKATALSADATb,P.TAHERPARVARa
Microwave-assisted hydrodistillation (MWHD), hydrodistillation (HD techniques were carried out for the analysis of volatile components of Stachys lavandulifolia Vahl from Iran. The oils obtained were analyzed by GC–MS. The extraction time while using the MWHD is no more than 24 min using a microwave power of 300 W. The major components by two methods of HD and MWHD were carvacrol and thymol in which (1.43, 2.63%) and (10.80, 8.14%) respectively.
Due to various usages of Stachys species or their oils and literature searches which indicated that the oil of Stachys lavandulifolia Vahl, have not been the MWHD in previous studies, we were interested in studying essential oil contents and compositions of Stachys species in Iran.
(Received July 15, 2011; accepted September 15, 2011)
Keywords: Stachys lavandulifolia Vahl Volatile compounds; Medicinal plants;
Gas chromatography–mass spectrometry
The genus StachyL.comprises more than 270 species  and is justifiably considered as one of the largest genera of the Labiatae. In the old world area there are two main centres of diversity for the genus, as assessed by the number and distribution of the species. One is confined to South and East Antolia, Caucasia, North West Iran and North Iraq, the other to the Balkan Peninsula . In Iran, 34 species of the this genus are present, among which, 13 are endemic 
.The plant is known as Chaye-kuhi in Iran and is a native plant, which has been used as an anxiolytic and sedative in Iranian folk medicine . However, the genus Stachys has been the subject of some phytochemical studies. Flavonoids, phenyl ethanoid glycosides, phenolic acids, iridoids, monoterpenes, sesquiterpenes, diterpenes, and triterpene saponins have been reported to be present in different Stachys species [5-10]. Stachys lavandulifolia has also been reported to contain volatile oil and a phenyl propanoid glycoside [11-12].
2. Experimental 2.1 Plant materials
About one kg of fresh aerial part of Stachys lavandulifolia Vahl at maturity was collected from agriculture college Garden of University, on June 2010. The dried aerial parts were stored in a dark place at 4°C.
2.2 Chemicals and Reagents
Helium, 99.999%, used as carrier gas, was purchased from Roham Gas Company (Tehran, Iran). Alkane mixture consisting of the C8-C20 alkanes (concentration of 40 mg/mL in hexane) was purchased from Fluka. All other chemicals were of the highest purity available from Merck or Fluka. Doubly distilled deionized water was used
2.3 Instruments and GC/MS Operating Conditions and procedure
Gas chromatography was performed with a Shimadzu model GC-17A (Kyoto, Japan) instrument equipped with a Shimadzu Quadropole-MS (qMS) model QP5050 detector. Separation was performed using a 30 m × 0.25 mm I.D capillary fused silica column 6 coated with a 0.25 μm film of DB5-MS (5% Phenyl-95% Polydimethyl Siloxane), and a split/splitless injector with a 1 mm internal diameter glass liner.
2.4 MWHD apparatus and procedure
MAHD was carried out with a Samsung microwave apparatus. The multimode microwave reactor has a twin magnetron (1000 W, 2455MHz) with a maximum delivered power of 1000W variable in 10W increments. A rotating microwave diffuser ensures homogeneous microwave distribution throughout the plasma coated cavity are35 cm × 35 cm × 35cm. Temperature was controlled by feedback to the microwave power regulator.
The experimental MADH variables have been optimized by the university method in Order to maximize the yield of essential oil. In a typical SFME procedure performed at atmospheric pressure, 60 g of fresh plant material was heated using a fixed power of 600 W for 24 min without added any solvent or water. A cooling system outside the microwave cavity condensed the distillate continuously. Condensed water was refluxed to the extraction vessel in order to provide uniform conditions of temperature and humidity for extraction. The extraction was continued at 100 °C until no more essential oil was obtained.
The sample (100 g of dried material was charged with a particle size of about 500 μm) was submitted to hydro-distillation for 1.5 h, using a Clevenger-type apparatus, according to the European Pharmacopoeia (1975). The volatile distillate was collected over anhydrous sodium sulphate and refrigerated until time of analysis. The yield of the oil was 3.1% v/w based on dry plant weight.
2.6 Qualitative and quantitative analyses
Most constituents were identified by gas chromatography through comparison of their retention indices (RIs) with those of the literature  or with those of authentic compounds available in our laboratories. The retention indices (RIs) were determined in relation to a homologous series of n-alkanes (C8–C24) under the same operating conditions. Further identification was made by comparison of their mass spectra on both columns with those stored in NIST 98 and Wiley 5 Libraries or with mass spectra from literature [13-15]. Component relative concentrations were calculated based on GC peak areas without using correction factors.
3. Results and discussion
The yields of the oils obtained from S. lavandulifoila by two methods of extraction were 0.82% and 0.45%. The yields of the oils extracted from other species were reported as 0.18%
from S. setifera and S. iranica, 0.18% from S. chrysantha, and 0.12% from S.candida . The S. lavandulifolia oil was examined by GC and GC-MS. The list of compounds identified in the oil
of S. lavandulifolia can be seen in Table 1. Fifty five compounds were identified, representing 93.50%, and 92.49% by HD and MWHD of the essential oils respectively, in which the major components were germacrene-D, β-phellandrene, β-pinene , myrcene , α-pinene and Z-β-ocimene . In a previous study the main components of the oil were reported to be spathulenol (35.0%) and caryophyllene oxide (25.6%), this finding was completely different from our study. The major component of the S. obliqua oil was germacrene-D, which was also one of the main components of the S. lavandulifolia oil. The main components of the oils of S. aegiptica (α-pinene) and S.
glutinosa (α-pinene and β-phellandrene) were presented as the major components of the S.
lavandulifolia oil  and . β-Pinene, one of the main components of S. recta and S. balansae oils, was present at an amount of 8.4% in S. lavandulifolia oil .
A qualitative comparison of the oil constituents of S. schtschegleevii with those of other Stachys species reported in the literature showed varying compositions [19, 22-24]. The oil of S.
Corsica from France consisted of carvacryl acetate (37.5%), Iinalool (13.4%), [alpha]-terpinyl acetate (7.7%) and [alpha]-terpineol (7.8%) (6). 1-Octen-3-ol (18.7%), linalool (11.0%), [alpha]- pinene (8.3%), [delta]-cadinene (5.0%), eugenol (4.3%), [beta]-selinene (4.3%), limonene (4.2%) and [beta]-pinene (4.1%) were reported as major components of S. athorecaltjx from Turkey .
Also, the oil of S. recta from Turkey contained 1-octen-3-ol (33.8%), linalool (13.0%) and [beta]- pinene (7.5%) . 1-Octen-3-ol was absent in the oil of S. balansae, but [beta]-caryophyllene (24.3%), [beta]-pinene (24.1%) and [alpha]-pinene (16.0%) were the major components . The same group also found that the oil of S. obliqua contains germacrene D (25.4%), thymol (16.4%), limonene (6.2%), borneol (4.9%), [alpha]-pinene (4.7%) and isomenthol (3.4%) (9). S. glutinosa oil from Corsica, France, contained terpinen-4-ol (13.1%), [alpha]-pinene (10.1%), [alpha]- terpineol (8.4%), [beta]-phellanderene (6.8%) and [alpha]-terpinene (6.1%) as major compounds . The major components of the oil of S. aegyptiaca from Egypt were [alpha]-pinene (54.46%), [beta]-caryophyllene (6.61%), limonene (5.35%) and myrcene (3.75%) .
In order to get access to the absolute mass percentage of the identified compounds, the essential oil of Stachys lavandulifolia Vahl was analyzed after extraction by hydro-distillation (HD) and MAHD.
3.1. Comparison of MWHD and HD for the analysis essential oil in Stachys lavandulifolia Vahl
The results in Table 1 show that the 26 compounds identified by HD were almost same with those by MWHD. As seen from Table 1, using the two different methods, the obtained relative contents for individual compounds (such as carvacrol) were very different. In the previous literatures [25-27], it has been demonstrated that microwave can much improve the extraction efficiencies of plant essential oil compounds [28-29]. This leads to the differences of the relative contents for individual compounds. Obviously, the HD method had good extraction efficiency.
Moreover, HD required 3 h to isolate the essential oil and organic solvent to perform further extraction. Under microwave irradiation, isolation of essential oil in fresh plant materials was rapidly completed by dry distillation. Due to the isolation, extraction and concentration performed in a single step, sample preparation needed only 24 min by using, MWHD. In microwave-assisted hydrodisti, distillation time was shorter than classical hydrodistillation and also the sample reached boiling stage more rapidly. This is an advantage of MWHD when it is compared to classical HD In conclusion; the present method is simple, rapid and effective and can be used for the analysis of volatile compounds in medicinal plants. As compared to conventional technique of HD, MWHD are simple, rapid for determination of essential oils in fresh Stachys lavandulifolia Vahl and other plant materials.
Table 1. Chemical composition of the essential oil from Stachys lavandulifolia Vahl
No Compound RI* HD (%) MWHD(%
1 α-Thujene 930 1.54 ─
2 α -pinene 939 19.66 ─
3 Sabinene 975 7.37 ─
4 β-pinene 979 2.30 ─
5 Myrcene 991 9.43 ─
6 Para Cymene 1025 0.91 ─
7 Limonene 1029 2.91 ─
8 β-Phellandrene 1030 14.31 ─
9 γ-Terpinene 1060 0.52 ─
10 Linalool 1097 1.45 ─
11 α -campholene 1120 0.48 ─
12 trans-Pinocarveol 1139 0.60 ─
13 TransLimonene oxide 1142 0.73 ─
14 4- Terpineol 1182 0.88 ─
15 Cryptone 1186 0.46 ─
16 α -Terpineol 1189 0.66 5.03
17 Myrtenal 1196 0.48 ─
18 Nerol 1230 ─ ─
19 Geraniol 1253 ─ ─
20 Thymol 1290 2.63 8.14
21 Carvacrol 1299 1.43 10.80
22 Cinnamyl alcohol 1304 ─ ─
23 γ-elemene 1338 ─ ─
24 α -Copaene 1377 0.67 ─
25 Geranyl acetate 1381 ─ 7.96
26 β -Bourbonene 1388 1.05 ─
27 trans-Caryophyllene 1419 ─ 5.12
28 Trans-α -Bergamotene 1435 0.58 ─
29 β -Farnesene 1443 ─ ─
30 β -Caryophyllene 1455 ─ ─
31 β-Farnesene 1457 2.92 1.11
32 Germacrene D 1485 3.43 1.10
33 Valencene 1496 ─ ─
34 Bicyclogermacrene 1500 5.22 0.88
35 β -Bisabolene 1506 ─ 1.96
36 γ -Cadinene 1514 ─ 2.05
37 Myristicine 1519 ─ 3.56
38 δ-Cadinene 1523 0.65 ─
39 elemol 1550 ─ 1.90
40 Spathulenol 1578 13.23 ─
41 Salvial-4(14)-en-1-one 1595 0.49 ─
42 Hexadecane 1600 0.59 0.56
43 10-epi-.gamma.-eudesmol 1624 ─ ─
44 δ-Cadinol 1636 ─ ─
45 Alloaromadendrene 1641 ─ 0.71
46 Cadinol 1650 ─ 0.61
47 β -Eudesmol 1651 ─ 0.86
48 α-Eudesmol 1654 ─ ─
No Compound RI* HD (%) MWHD(%
49 Bisabolol oxide B 1658 ─ ─
50 Bisabolone oxide 1685 ─ ─
51 α -Bisabolol 1686 0.61 ─
52 Heptadecane 1700 ─ 17.77
53 Bisabolol oxide A 1749 ─ 2.55
54 Benzyl benzoate 1760 0.91 ─
55 Geranyl linalool isomer 2004 ─ 2.11
*RI= retention indices in elution order MD%=microwave distillation area %
In the work, extraction and determination of essential oil of Stachys lavandulifolia Vahl by HD and MWHD extraction methods were successfully performed. It has been shown that isolation; extraction and concentration of essential oil in fresh Stachys lavandulifolia Vahl can be done by two methods separately. Forty seven compounds were identified in the Stachys lavandulifolia Vahl by using the proposed methods. The experimental results demonstrate that using much less sample amount, shorter extraction time and simpler procedure, MWHD methods can achieve comparable results with those by HD for determination of essential oils in fresh materials. The major component by two methods were carvacrol and thymol in which (1.43, 2.63%) and (10.80, 8.14%), respectively. The major advantages of MWHD as compared with HD and reported HD are the low cost and highest extraction efficiency.
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