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A correlation between wet cupping therapy treatment and Serum lipids profile levels in Thi-Qar governorate –Iraq

Mohammad Q. Sultan

College of Dentistry, University of Al – Ayen, Thi-Qar, Iraq [email protected]

Abstract:

Serum samples,5 ml of venous blood collected before and after cupping from each person. The serum was isolated and processed for subsequent examination at 20 °C. Serum total cholesterol, HDL, LDL, VLDL and TG levels.

The results showed that there was a relative decrease (p≤0.05) in the group (2) total cholesterol level 147.5 ±23.47 mg/dl after cupping as compared to venous blood sample160.75 ±22.33 mg/dl before of cupping.

Blood sample after cupping, it noted that there was a relative decrease in Triglyceride level 102.5

± 53.24 mg/dl as compared to venous blood sample125.62 ±53.58mg/dl before of cupping.

In the case of high-density lipoprotein (HDL), there was a relative increase 54.9-±15.11 mg/dl in human blood concentration after cupping as compared to venous blood sample39.06 ±9.08mg/dl before of cupping.

Blood sample from cupping showed highly significant decrease in low-density lipoproteins level72.2 ±31.67 as compared to venous blood sample96.56 ± 21.62 mg/dl before of cupping.

Introduction:

Cupping therapy is an ancient healing methodwhich applied to selected skin points and sub- atmospheric pressure produced, either by heat or by suction [1, 2].

The cupping mechanism of action defined by several hypotheses which till now is not clear

[3].The theory of immunomodulation was suggested by [10], indicating that the same mechanisms of action exist for cupping and acupuncture. The immunomodulation theory suggests that by stimulating the skin, manipulating the microenvironment could transform the neuroendocrine- immune system into biological signals and activate it [10]. In general, the effects of sub- atmospheric pressure suction, facilitating peripheral blood circulation, and improving immunity likely played a major role in these mechanisms[4]. On the other hand, the effects of cupping therapy involve an increase in blood flow to the skin[5], a shift in the biomechanical properties of the skin[6], an increase in pain thresholds, an improvement in local anaerobic metabolism[7], a decrease in inflammation[8], and cellular image modulation.Furthermore, cupping therapy will help in cardiovascular disorders, immune system disorders, and metabolic diseases such as migraine, low back pain, fibromyalgia, shoulder pain, recurrent nonspecific neck pain, angina, arthritis, high blood pressure, myocardial ischaemic and inflammatory conditions, herpes zoster, Behc'et disorder, secondary amenorrhea, depression and anxiety, fatigue [9].

It is worth noting that Hyperlipidemia classified into two subcategories,a hypercholesterolemiathat caused for atherosclerosis and cardiac attacks, and a pancreatitis- responsible hypertriglyceridemia. In patients with hyperlipidemia who blood transfusion antihyperlipidemic medication, serum total cholesterol, LDL cholesterol, and triglyceride levels decreased almost twice as much as in patients treated with antihyperlipidemic medication alone

[11].

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The present work will describe the effect of cupping treatment on the level total lipid (i.e.

Total cholesterol, Triglyceride, HDL, LDL and VLDL) on volunteers on Thi-Qar governorate – Iraq.

Material and Methods:

Sixty men (30-45 years of age) were included in the study; patients who underwent wet cupping were fasted for 12-14 hours and the remainder of the study that not taking any antihyperlipidemic medication or high-calories diet intake. Wet cuppingperformed by a skilled person in one of the private cupping clinics in the Thi-Qar governorate. That include incisions (7 incisions with an average depth of 4 millimeters and a length of 1 centimeter) and vacuumed in interscapular region for 3 minutes used for the hygienic procedure. On average, each patient received a cumulative quantity of 40 ml of blood.

Samples Blood Collection

Serum samples: 5 ml of venous blood collected before and after cupping from each person. The blood was able to stand at 27 Cͦ and after that centrifuged for 15 minutes at 2900 rpm. The serum was isolated and processed for subsequent examination at 20 °C. Serum cholesterol; HDL, LDL, VLDL and TGcalculated using the Biolabo Company (France) enzymatic kit system [12]. The equation (Friedwald formula) used to measure indirect serum LDL cholesterol: (TG/5)[13].

Statistical Analysis

To calculate the expressionof the mean± standard deviation. The Social Science Software Statistical Package (SPSS) for Windows version 10.0.0. used to perform all statistical studies. As statistically relevant, (p <0.05) was recognized.

Results

For the findings concerning the serum lipid profile shown in Table (1), The results showed that there was a relative decrease (p≤0.05) in the group (2) total cholesterol level147.5 ±23.47 mg/dl after cupping as compared to venous blood sample160.75 ±22.33 mg/dl before of cupping.

Blood sample aftercupping, it noted that there was a relative decrease in Triglyceride level 102.5

± 53.24 mg/dl as compared to venous blood sample125.62 ±53.58mg/dl before of cupping.

In the case of highdensity lipoprotein (HDL), there was a relative increase 54.9-±15.11 mg/dl in human blood concentration after cupping as compared to venous blood sample39.06 ±9.08mg/dl before of cupping.

Blood sample from cupping showed highly significant decrease in low density lipoproteins level72.2 ±31.67 as compared to venous blood sample96.56 ± 21.62 mg/dl before of cupping.

Finally, the results showed a relative decrease in concentration VLDL level 20.4 ±10.45mg/dl after cupping as compared to venous blood sample25.12 ±10.17mg/dl before of cupping.

Table (1) Showed Serum Lipid Profile ConcentrationsBefore and afterthe process

Lipid profile Group (1)

Group (2) LSD

Before therapy After therapy

Total Cholesterol 160.75

±22.33 147.5 ±23.47

16.37

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HDL 39.06

±9.08 54.9 ±15.11

8.91

[mg / dl] ( mean , ±SD)

LDL 96.56 ±

21.62 72.2 ±31.67

19.38

[mg / dl] ( mean , ±SD)

VLDL 25.12

±10.17 20.4 ±10.45

7.56

[mg / dl] ( mean , ±SD)

Figure (1) Showed Serum Lipid Profile Concentrations Before and after cupping therapy.

Discussion

The results of the current study showed a significant decrease in total cholesterol, LDL- cholesterol, triglyceride, and LDL after cupping compared to before cupping (p ≤ 0.05).

Although serum HDL increases. Cholesterol and VLDL concentrations in blood highly level correlated with the incidence of ischemic heart disease all over the world. Here comes the significance of cupping therapy with respect to reducing heart disease by raising the concentration of new cholesterol and decreasing harmful cholesterol and triglycerides. Wet cupping may also be one of the ways of improving the lipid profile in patients with diabetes, thus acting as an important tool for improving the metabolism of lipids and thus preventing the production of atherosclerosis, which is in agreement with other studies[14, 15]. A common type of male anxiety and depressive disorders is dyslipidemia [16, 17]. The decrease in lipid profile parameters in males in resent study suggested that wet cupping used to reduce the symptoms of depression. These results of previous study that showed that cupping to help psychological well- being by reduce anger[18].The lipid profile reduced in the men in recent study and that enhance the health of cardiac activity in long term.

160.75 125.62 39.06 96.56 25.12

147.5 102.5 54.9 72.2 20.4

T O T A L C H O L E S T E R O L

T R I G L Y C E R I D E H D L L D L V L D L

Before Cupping After Cupping

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